发明名称 |
METHOD OF DISCRIMINATING AT LEAST TWO CELL POPULATIONS, AND APPLICATION |
摘要 |
Discrimination and counting of populations of biological elements, optionally present in a sample, comprises simultaneous marking of the populations by three different probes; measurement of the total quantity (qNCM) of non-covering marker; measurement of the total quantity (qCM) of covering markers; formation of the ratio (R) i.e. qNCM to qCM; formation of a diagram presenting the ratio (R) of the total quantity of biological elements marked by the covering marker; and/or quantification of the populations. Discrimination and counting method of at least two populations of biological elements having specific biological characteristics, optionally present in a sample, comprises simultaneous marking of the populations by three different probes, (made) detectable by three different markers of which two (covering markers or CM) presenting an emission spectrum, overlapping emission spectra and the third (non-covering marker or NCM) presenting an emission spectrum not overlapping the spectra of two other markers (non-covering emission spectrum); measurement of the total quantity (qNCM) of NCM, in a detection band in the non-covering marker emission spectrum; measurement of the total quantity (qCM) of CM in a common detection band with the emission spectra of covering markers; formation of the ratio (R) i.e. qNCM to qCM; formation of a diagram presenting the ratio (R) according to the total quantity of biological elements marked by the covering marker; and/or quantification of the populations of the biological elements present on the diagram. |
申请公布号 |
CA2632343(A1) |
申请公布日期 |
2007.07.19 |
申请号 |
CA20062632343 |
申请日期 |
2006.12.05 |
申请人 |
HORIBA ABX SAS |
发明人 |
LEFEVRE, DIDIER;LACOMBE, FRANCIS;VERIAC, SYLVIE;BELLOC, FRANCIS |
分类号 |
G01N33/569;G01N15/14;G01N33/50;G01N33/58 |
主分类号 |
G01N33/569 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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