| 摘要 |
FIELD: medicine. ^ SUBSTANCE: the present innovation deals with creating anticoagulant preparations of animal origin, moreover, it deals with the one being a fragment of collagen at molecular weight of about 12 kDa containing about 10.0-12.0% carbohydrates, 6.0-7.5% sulfate groups, macro- and microelements. The method for obtaining this preparation deals with homogenization, extracting with distilled water at pH being 8.0-8.5 followed by treating the extract with enzymatic preparation at about 35-37°C at pH being 7.5-8.5 for 3.5-4.5 h, then it is necessary to add ethanol to the mixture cooled up to room temperature up to its content in the extract of 40% and then the developed filamentous residue should be separate, protein fibers should be pressed upon a filter and successively washed with 40%- and 96%-ethanol and dried, then the product should be dissolved in 0.05-0.1 M sodium bicarbonate solution, then one should repeat fermentolysis under the same conditions; the solution obtained should be supplemented with ethanol up to its final concentration of 50%; the residue should be pressed upon a cloth filter, washed with 50%-ethyl alcohol solution in distilled water, and then - with 96%-ethyl alcohol to be dried on air; then the product should be dissolved in water and deposited with acid, the residue should be separated due to centrifuging, dissolved in sodium bicarbonate, then protein-containing solution should undergo dialysis or ultrafiltration upon a membrane being permeable for substances at molecular weight being 15 kDa, after that desalinized protein solution should be separated by passing it through a sterilizing membrane at pore size of 0.2 mcm, then membrane-passing protein fraction should be collected and the target product should be dried. The innovation widens the range of substances of another mechanism of anticoagulant action against the action of heparin and, also, decreases the quantity of side effects and extends raw material basis for obtaining preparations of anticoagulant activity. ^ EFFECT: higher efficiency. ^ 3 cl, 6 dwg, 7 ex, 2 tbl |
