New isolated nucleic acid, useful as source of primers for selective detection, in plants and soil, of the AG1-IB subgroup of Rhizoctonia solani

摘要

An isolated nucleic acid (I), and its fragments, complements, homologs and variants are new. New isolated nucleic acid (I) is: (A) Seq. (1) of 1547 nt, or especially its fragments Seq. (2)-(132), all reproduced, and their complements; (B) sequences that hybridize to (a) under stringent conditions; (C) sequences sufficiently homologous with (a) or (b) to be their functional analogs; (D) sequences that are equivalent to (a)-(c) within the degeneracy of the genetic code; and (E) sequences of (a)-(d) modified by deletion, addition, substitution, translocation, inversion and/or insertion, provided that they remain functionally equivalent, also fragments of such sequences. Independent claims are also included for the following: (1) primers that consist of fragments of Seq. (2)-(132), for use as forward primer, or their complements, for use as reverse primer; and (2) fungal detection kit, especially for 'salad blight', that contains at least one primer of (1) and optionally instructions.