Regenerated corneal endothelial cell sheets, processes for producing the same, and methods of using the same

摘要

An improved process for producing a regenerated corneal endothelial cell sheet, comprising the steps of allowing corneal endothelial cells collected from a tissue to be cultivated on a cell culture support having its surface covered with a polymer of which the hydrating force varies in a temperature range of 0-80° C., and after the culture, ( 1 ) adjusting the temperature of the culture solution to the temperature at which the polymer on the substrate surface is hydrated, ( 2 ) bringing the cultured corneal endothelial cell sheet into close contact with a carrier, and ( 3 ) detaching the sheet together with the carrier. The regenerated corneal endothelial cell sheet obtained by the process will adhere very well to living tissues.