| 摘要 |
<p>The present invention relates to a production method of an enantiomer-enriched compound, which includes the following (1) and (2):
(1) contacting an enantiomer mixture with a stereoselective dehydrogenase, for which NAD+ (nicotinamide adenine dinucleotide) is a coenzyme, thereby preferentially oxidizing one enantiomer and leaving the other enantiomer to form NADH, and
(2) contacting the NADH formed by the oxidation reaction in (1) with a water-forming NADH oxidase stable under oxidative conditions to convert the NADH to NAD+.</p><p><Paragraphs id="pa02" num="0002" xmlns:base="http://www.sipo.gov.cn/XMLSchema/base">According to the present invention, in the production of an enantiomer-enriched compound from an enantiomer mixture, which uses a dehydrogenase, NAD+ can be regenerated even without addition of a stabilizer and an enantiomer-enriched compound can be obtained efficiently.</p> |
