| 摘要 |
FIELD: biotechnology, microbiology. ^ SUBSTANCE: method involves selection of damaged organs from dead nutrias from local epizootic focus and preparing suspension from these organs. Then method involves inoculation in differential diagnostic media and isolation of pure cultures of pathogens. Cultures Streptococcus pneumoniae and Streptococcus faecalis are grown in beef-extract broth with addition glucose to final 0.2% concentration and with titer value 4-5 billions microbial cells/1 cm3 and inactivated by addition of formalin to final concentration 0.4-0.6% and kept at temperature 37°C for 72-96 h. Cultures are mixed in equal ratios, aluminum hydroxide solution is added in the amount 15% to volume, stirred thoroughly and sealed. Method provides preparing harmless, highly immune and highly specific vaccine. ^ EFFECT: improved preparing method. ^ 1 tbl, 1 ex |
