摘要 |
FIELD: veterinary medicine. ^ SUBSTANCE: method involves taking animal blood samples, producing erythrocyte suspension by mixing blood and physiologic salt solution and centrifuging it. Reaction is carried out on 96-cell plastic flat-bottom tablet. Erythrocyte suspension and immunospecific sera are introduced into the cells, shaken, placed into thermostat and reading data. The tablets are shaken with tablet-type photometer. Visible light absorption capacity in bandwidth of 630-650 nm is determined in samples by means of the tablet-type photometer before placing the tablet into thermostat. The samples are incubated at 37°C in thermostat for 40 min. Then, the samples are additionally shaken and incubated for more 1.5-2.0 h, and visible light absorption capacity in bandwidth of 630-650 nm is determined once more. The reaction readings are taken by subtracting visible light absorption indices of the samples after incubation from those before incubation. The received values are transformed into serologic test points. ^ EFFECT: high estimation accuracy; facilitated animal testing process. ^ 6 tbl |