| 摘要 |
The invention concerns a method having the steps of: combining an aqueous fermentation broth containing ansamitocins with a water-immiscible solvent and a water-soluble solvent to form an organic layer and an aqueous layer, wherein: (i) the water-immiscible solvent comprises at least 70 % by volume of at least one solvent that is ethyl acetate, n-butylacetate, isopropylacetate, methyl ethyl ketone (MEK) methyl t-butyl ether, toluene, xylene or a mixture thereof, (ii) the water-soluble solvent comprises at least one solvent that is C<SUB>1-5</SUB> alcohol, tetrahydiofuran, acetonitrile, or a mixture thereof, (iii) the ratio of the water-immiscible solvent to water-soluble solvent is in the range of 10:1 to 2:1 by volume, and (iv) the ratio of total solvent to fermentation broth is at least 2:1 by volume; partitioning the ansamitocins into the organic layer; separating the organic layer from the aqueous layer; and removing at least a portion of the solvent from the organic layer to provide an organic extract that comprises ansamitocins. The extract can be further manipulated to produce purified ansamitocins. |
