| 摘要 |
PROBLEM TO BE SOLVED: To provide a method for real-time examination of a gene base substitution by the kind of emitted fluorescence by reacting a specific sequence incorporated into an allele-specific primer for identifying a specific site of a gene with a fluorescent labeling probe. SOLUTION: The method for real-time examination of a gene base sequence comprises binding an oligonucleotide having a specific base sequence to the 5' side of an allele-specific primer corresponding to each base substitution of the gene, mixing the oligonucleotide with a fluorescent labeling probe having the same base sequence, carrying out a polymerase chain reaction (PCR reaction) and detecting fluorescence emitted by PRET (Fluorescence Resonance Energy Transfer) effect of the fluorescent labeling probe. COPYRIGHT: (C)2007,JPO&INPIT
|
