| 摘要 |
PURPOSE:To easily obtain alpha-1,3-glucanase useful for the decomposition of sordes on tooth, etc., on an industrial scale,by culturing a specific bacterial strain of Bacillus genus in a medium. CONSTITUTION:Microorganisms capable of decomposing and utilizing insoluble glucan as a carbon source are identified to obtain Bacillus circulans BC-8 having a cell size of 0.5X1.3-2.6mum, exhibiting microbial properties such as Gram-positive bacillus and capable of producing alpha-1,3-glucanase which is an enzyme capable of specifically cutting and decomposting alpha-1,3-glucoside bond of 1,3-alpha-D- glucan existing in sordes on tooth, etc. The bacterial strain is cultured in a medium containing 0.1W/V% of insoluble glucan and peptone, etc., at 30-37 deg.C and pH 6-7 for a desired period and the cultured liquid is subjected e.g. to centrifugal separation to recover alpha-1,3-glucanase having the following physical and chemical properties. Optimum pH, 4-9; optimum temperature, 45-50 deg.C; inhibited by Hg<2+>, etc.; mol.wt., 180,000 (SDS-polyacrylamide gel electrophoresis); etc. |
