发明名称 |
Midler og fremgangsmåder til fremstilling af en protein gennem chromatinåbnere, som kan göre chromatin mere tilgængeligt for transkriptionsfaktorer |
摘要 |
Described are means and methods for providing a cell with a protein expression unit, the method comprising providing a nucleic acid sequence comprising the unit with a nucleic acid sequence encoding a binding site for a member of a chromatin modification system for rendering chromatin more accessible for transcription (opener), wherein the opener is present in the cell. Preferred openers comprise histone modification proteins, chromatin remodeling proteins and trithorax group proteins or equivalents. The cells thus generated and nucleic acid sequences encoding such openers are provided. Openers are preferred in the context of STAR and TRAP sequences. |
申请公布号 |
DK1572994(T3) |
申请公布日期 |
2007.05.29 |
申请号 |
DK20030782989T |
申请日期 |
2003.12.19 |
申请人 |
CHROMAGENICS B.V. |
发明人 |
KWAKS, THEODORUS, HENDRIKUS, JACOBUS;OTTE, ARIE, PIETER;SEWALT, RICHARD, GEORGE, ANTONIUS, BERNARDUS |
分类号 |
C12N9/10;C12N15/63;C12N15/85 |
主分类号 |
C12N9/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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