| 摘要 |
The invention relates to a method for characterising, classifying and distinguishing tissues and cell types, for predicting the behaviour of tissues and groups of cells, and for identifying genes which have altered in their expression. Said method is characterised in that the base cytosine (not 5-methyl-cytosine) in a genomic DNA taken from any tissue sample is converted into uracil through treatment with a bisulphite solution. Fractions of the genomic DNA which has been treated are amplified by using very short or degenerated oligonucleotides, and the remaining cytosines of the amplified fractions are detected by means of hybridisation or polymerase reaction. The data generated from the analysis and automatically transferred to a processing algorithm is then used to draw conclusions as to the phenotype of the cell material which was analysed.
|
