METHOD FOR DETERMINATION OF UGT1A1 GENE POLYMORPHISM

摘要

Disclosed is a method for simultaneous determination of two or more polymorphisms associated with UDP-glucuronosyltransferase 1A1 (UGT1A1) gene. The method comprises the steps of: amplifying both of a gene region having a polymorphic site associated with UGT1A1*28 and at least one gene region having a single nucleotide polymorphism (SNP) in a gene amplification reaction; and digesting the resulting amplification fragments with a restriction enzyme and then subjecting the digestion products to capillary electrophoresis, wherein two or more polymorphisms are determined simultaneously based on the results of the capillary electrophoresis. Similarly, the polymorphism of a gene other than UGT1A1 gene can also be determined rapidly, in a simple manner and with good accuracy.