发明名称 |
ADENO-ASSOCIATED VIRUS WITH INVERTED TERMINAL REPEAT SEQUENCES AS PROMOTER |
摘要 |
Described herein are constructions of recombinant DNA comprising modified adeno-associated virus (AAV) DNA sequences capable of functioning as a eukaryotic expression vector for expressing foreign DNA sequences using a novel transcription promoter comprising the termini of AAV DNA. It is shown that expression of a test reporter gene can be obtained from this vector in mammalian cells. It is further shown that this combination of vect or and promoter can be used to introduce and express a human gene and correct a genetic defect in human cells resulting from malfunction of the mutant endogenous gene. Further, the vector can be used to correct the genetic defect by expressing a modified version of the human gene consisting of a fusion of part of the said gene and a synthetic sequence contained in the vector.</SDOA B>
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申请公布号 |
CA2136441(C) |
申请公布日期 |
2007.04.24 |
申请号 |
CA19932136441 |
申请日期 |
1993.06.02 |
申请人 |
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发明人 |
CARTER, BARRIE J.;FLOTTE, TERENCE;AFIONE, SANDRA;SOLOW, RIKKI |
分类号 |
C12N15/86;A61K38/00;A61K48/00;C07K14/47;C12N5/10;C12N15/12;C12N15/62;C12N15/864 |
主分类号 |
C12N15/86 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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