METHOD FOR PLANT GENOME SITE-DIRECTED MODIFICATION

摘要

Provided is a method for plant genome site-directed modification. Specifically, a method for plant genome site-directed modification introduced by RNA is provided. By utilizing nucleic acid construct with particular structure, site-directed modification may be performed at pre-determined site in plant genome with high efficiency. Useful for screening plant with improved traits efficiently.

主权项

1. A targeted modification method for plant genome, comprising the steps of:
(a) introducing a nucleic acid construct expressing chimeric RNA and Cas protein into a plant cell to obtain a transformed plant cell, wherein the chimeric RNA is a chimera consisting of CRISPR RNA (crRNA) specifically recognizing targeted sites to be modified (or to be cut) and trans-activating crRNA (tracrRNA); and (b) under suitable conditions, forming chimeric RNA (chiRNA) through transcription of said nucleic acid construct in the transformed plant cell and expressing said Cas protein in said transformed plant cell, so that, in said transformed plant cell, targeted cleavage on genomic DNA is conducted by Cas protein under the guidance of said chimeric RNA, thereby performing targeted modification on genome.