| 发明名称 |
TRANSFORMED HUMAN PLURIPOTENT STEM CELLS AND ASSOCIATED METHODS |
| 摘要 |
The present disclosure provides transformed human pluripotent stem cell (t-hPSC). t-hPSCs are not dependent on Oct4 for renewal and survival, however exhibit a sensitivity to reduced levels of the transcription factor Nanog. Also provided are methods of culturing cells for use in a cell-based screening assay comprising placing one or more transformed human pluripotent stem cells into a receptacle and culturing said stem cells in the receptacle to form a monolayer of stem cells without cell overlap. Methods of screening compounds using t-hPSCs are also described. |
| 申请公布号 |
US2016264935(A1) |
申请公布日期 |
2016.09.15 |
| 申请号 |
US201615164528 |
申请日期 |
2016.05.25 |
| 申请人 |
McMaster University |
发明人 |
Bhatia Mickie;Werbowetski-Ogilvie Tamra;Sachlos Eleftherios;Fischer Russell Daniela;Laronde Sarah;Lee JungBok;Szabo Eva;Risueno Ruth |
| 分类号 |
C12N5/0735;C12Q1/04;G01N33/50 |
主分类号 |
C12N5/0735 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A transformed human pluripotent stem cell (t-hPSC) wherein the cell does not require bFGF for maintenance of an undifferentiated state. |
| 地址 |
Hamilton CA |
