Enone reductase and methods of making and using thereof

摘要

An isolated enone reductase characterized by a molecular mass of 61300+/-5000 Da; NADPH and NADPH as co-factor; a temperature optimum of 55-60° C. at pH 7.4; a pH optimum of 4.5-8.5 and substrate specificity on ∝,beta-unsatured ketones; a process for producing it from a microorganism and a process for producing levodione from ketoisophorone using such reductase.