TEMPERATURE-TRIGGERED IMMOBILIZATION AND PURIFICATION OF ANTIBODIES

摘要

This invention provides compositions and methods for capture and purification of antibodies. Protein sequences with inverted solubility temperature profiles are fused to antibody-binding proteins. Fusions of elastin-like peptides (ELPs) and protein G and/or protein L can be readily purified and employed to capture and purify immunoglobulins. To avoid the drawbacks of conventional affinity chromatography, ELP fusion proteins consisting of an elastin-like polypeptide made up of the pentapeptide VPGVG repeating units and Ig-binding ligands, protein A, protein G, protein L, and hybrid ligands such as protein LG are used for immobilization and purification of monoclonal or polyclonal antibodies from a material containing these antibodies by simple temperature transition cycling.