发明名称 |
METHOD OF DETECTING DNA BY DNA HYBRIDIZATION METHOD WITH THEUSE OF FLUORESCENT RESONANCE ENERGY TRANSFER |
摘要 |
A practically usable DNA detection method by a DNA hybridization method with the use of fluorescent resonance energy transfer wherein the background luminescence (i.e., luminescence based on the luminescence of a donor and th e direct excitation of an acceptor) affecting the sensitized luminescence of the acceptor is minimized so that a target DNA strand can be conveniently and very easily detected in a homogeneous solution. In this DNA detection method, a DNA is detected by the DNA hybridization method by using, as detection reagents, streptoavidin labeled with a fluorescent rare earth metal complex, a nucleic acid probe modified with biotin and a nucleic acid probe labeled with an organic cyanine pigment and taking advantage of fluorescent resonance energy transfer. |
申请公布号 |
CA2397545(C) |
申请公布日期 |
2007.04.03 |
申请号 |
CA20002397545 |
申请日期 |
2000.07.26 |
申请人 |
JAPAN SCIENCE AND TECHNOLOGY CORPORATION |
发明人 |
MATSUMOTO, KAZUKO;SUEDA, SHINJI |
分类号 |
C12Q1/68;G01N33/50;C07D209/08;C12N15/09;G01N33/53;G01N33/566;G01N33/58 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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