| 摘要 |
An herbicidal substance, elemicin, purified from Asarum sieboldii Miq. and a preparation method thereof are provided to serve a novel herbicide having different chemical structure compared with conventional herbicides, so that it will be a lead compound for development of new herbicides. The method for preparing the elemicin having herbicidal effects purified from Asarum sieboldii Miq. comprises the steps of: (a) extracting Asarum sieboldii Miq. with methanol and drying the extract; (b) dissolving the methanol extract of Asarum sieboldii Miq. in hexane to prepare a hexane fraction; (c) subjecting the hexane fraction to column chromatography using hexane-ethylacetate-methanol solution(5:1:0.1 v/v/v) to obtain HB fraction; (d) subjecting the HB fraction to column chromatography using hexane-ethylacetate-methanol solution(9:1:0.5 v/v/v) to obtain HBB fraction; (e) subjecting the HBB fraction to column chromatography using hexane-ethylacetate solution(5:5 v/v) to obtain HBBA fraction; and (f) subjecting the HBBA fraction to affinity column chromatography using methanol-acetonitrile solution(9:1 v/v) to obtain HBBAA fraction.
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