Novel isoamylases and associated methods and products

摘要

Methods are provided for distinguishing between different isoamylase genes of individual genomes of a polyploid plant. These methods enable a polyploid plant to be produced and/or identified which has at least two impaired isoamylase genes. In particular, the methods enable various plants of a polyploid species to be produced and/or identified which, between the plants, lack a functional isoamylase gene for each genome of the plant. Such plants can be cross-bred to produce a plant that lacks any functional isoamylase protein. Furthermore, numerous isoamylase genes, proteins and cDNA sequences from various polyploid plant species, including hexaploid wheat are provided