Fluorescent substrates for detecting organophosphatase enzyme activity

摘要

Disclosed are compounds of the formula (I): wherein R³, R⁴, R⁵, R⁹, and R¹⁰ are selected from the group consisting of H and groups or atoms other than H, and R⁶ and R⁸ are halo or hydrogen; X¹, X², and X³ are independently O or S; provided that R⁹ and R¹⁰ are not simultaneously H, when all of X¹, X², and X³ are O; and of the formula (II) wherein R¹¹-R¹⁴ are selected from the group consisting of H and groups or atoms other than H; X⁴-X⁹ are independently O or S; n and m are 0 or 1 but m and n cannot be 0 simultaneously; R¹⁵-R²⁴ can be H or any substituent so long as the compound of formula II upon hydrolysis provides a fluorescent compound. These compounds are useful as substrates with high specificity for organophosphatase particularly human paraoxonase and bacterial organophosphorus hydrolase. Also disclosed is a method for detecting and/or measuring the paraoxonase activity in a fluid comprising contacting the fluid with a fluorescent substrate and measuring the fluorescence of the fluorescent product formed.