FED-BATCH FERMENTATION PROCESS AND CULTURE MEDIUM FOR THE PRODUCTION OF PLASMID DNA IN E.COLI ON A MANUFACTURING SCALE

摘要

A process for producing plasmid DNA E. coli cells comprises a pre-culture and fed-batch process. The culture media of the batch phase and the culture medium added during the feeding phase are chemically defined. The culture medium of the feeding phase contains a growth-limiting substrate and is added at a feeding rate that follows a pre-defined exponential function, thereby controlling the specific growth rate at a pre-defined value. The process results in high yield and homogeneity of plasmid DNA.