发明名称 |
METHYLATION SPECIFIC PRIMER EXTENSION ASSAY FOR THE DETECTION OF GENOMIC IMPRINTING DISORDERS |
摘要 |
<p>Provided is a method for determining genomic imprinting disorders in a patient based upon methylation specific primer extension in a format amenable to high throughput and multiplex formats. After bisulfite modification of a genomic sample, DNA is amplified and hybridized to discrimination primers specific for a CpG dinucleotide site in the sample. Because no extension products from the discrimination primers are produced from DNA that has a deletion or functional inactivation at the CpG site, the sample may be diagnosed as having a genomic imprinting disorder by way of comparison with a normal sample.</p> |
申请公布号 |
WO2007015993(A1) |
申请公布日期 |
2007.02.08 |
申请号 |
WO2006US28516 |
申请日期 |
2006.07.21 |
申请人 |
BAYER HEALTHCARE LLC;KOEHLER, RUTH;BEARD, CHRIS |
发明人 |
KOEHLER, RUTH;BEARD, CHRIS |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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