发明名称 |
MODIFIED SINGLE-STRANDED DNA-BINDING PROTEIN DERIVED FROM EXTREMELY THERMOPHILIC BACTERIUM, AND METHOD FOR ISOTHERMALLY AMPLIFYING NUCLEIC ACID BY USING THE PROTEIN |
摘要 |
PROBLEM TO BE SOLVED: To establish a technique by which nonspecific amplification is controlled in the nucleic acid amplification in an isothermal amplification reaction to improve amplification accuracy. SOLUTION: The modified single-stranded DNA-binding protein derived from an extremely thermophilic bacterium has an amino acid sequence exhibiting a function contributing to the improvement of amplification efficiency of a template nucleic acid in an isothermal amplification system using a chain-substituting polymerase. The protein has a modified site at which at least one of modification of deletion, substitution, addition and insertion of one or more amino acids is caused. COPYRIGHT: (C)2007,JPO&INPIT
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申请公布号 |
JP2007031406(A) |
申请公布日期 |
2007.02.08 |
申请号 |
JP20050220580 |
申请日期 |
2005.07.29 |
申请人 |
AISIN SEIKI CO LTD;INSTITUTE OF PHYSICAL & CHEMICAL RESEARCH |
发明人 |
SHIGEMORI YASUSHI;SHIBATA TAKEHIKO;MIKAWA TSUTOMU |
分类号 |
C07K14/195;C12N15/09;C12Q1/68 |
主分类号 |
C07K14/195 |
代理机构 |
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