IN VITRO MODEL FOR HEPATITIS C VIRION PRODUCTION

摘要

The invention is related to a construct comprising a hepatitis C virus (HCV) nucleic acid sequence that comprises from 5€ to 3€ on the positive-sense nucleic acid a 5€ untranslated region (UTR), a full-length open reading frame (ORF) encoding an HCV polyprotein whose cleavage products form functional components of HCV virus particles and RNA replication machinery, and a 3€ untranslated region (UTR), said sequence being infectious, and additionally, a ribozyme pair positioned to generate the 5€ and 3€ ends of said sequence when cleaved, and related methods of making and methods of using.