| 摘要 |
FIELD: medicine, bacterial infections. ^ SUBSTANCE: invention relates to problem for improving prophylaxis and treatment of plague. In addition to the existing first model caused by infection of animal with suspension of virulent culture of pathogen in the 0.85% sodium chloride isotonic solution the method involves the development of the second model, namely, the infectious-toxic model caused by infection of mice with suspension of cells in human hemolyzed erythrocytes after incubation at 37°C for 3 h. Then results after analysis of effectiveness of antibacterial preparations or agents for passive immunotherapy are compared in two models by values ED50, the protection index, and formation of immunity - by values LD50 for cultures, the immunity index and the immunity overcoming index. The infectious-toxic plague model is created by infection of mice with bacterial cells after activation of their toxic substances - "murine" toxin and lipopolysaccharide - in the following sequence: from 24 h agar culture of virulent plague microbe strain the cell suspension is prepared in the cell concentration 109 cells/ml in 0.85% sodium chloride isotonic solution. Then this solution is diluted by 10-fold and necessary amounts of microbial cells in minimal volume are added to human hemolyzed erythrocytes in the ratio 1:10 up to achievement of required concentrations of microbial cells in volume 0.1 ml used for subcutaneous infection of mice wherein the infection doses for formation of the infectious process in two models are similar. Activation of plague microbe toxic substances provides attainment the maximal development of virulence of pathogen by LD50 values and infection caused by this pathogen show the more transient profile wherein the average span life of dead animals is reduced by 1.5-3 days, and reliability of differences is estimated to be 95-99%. Using the proposed method provides preparing the additional information for more precise prognostication of effectiveness of the corresponding preparations in their using in stage of infectious-toxic shock, and for study of capacity of novel vaccine preparations in formation of antitoxic immunity. Invention can be used in selection of the more effective antibacterial and vaccine preparations and agents for passive antitoxic immunotherapy of the plague infection. ^ EFFECT: improved method for prognostication. ^ 2 cl, 4 tbl, 3 ex |
