Chimeric skin comprised of human immortalised keratinocyte cells and human donor cells for use in skin grafting and plastic surgery methods

摘要

Disclosed is a combination of in vitro cultured conditionally immortal carrier human keratinocytes and donor patient-derived keratinocytes to form skin grafts. Such carrier keratinocytes derived from in vitro cell culture sources can be cocultured with donor patient keratinocytes using monolayer or organotypic culture methods to produce an engineered chimeric skin suitable for grafting. A conditionally immortal keratinocytes are keratinocytes that are immortal under defined growth conditions. A keratinocyte is considered immortal if it can be cultured under the defined growth conditions for more than 50 passages. The in vitro cultured conditionally immortal carrier human keratinocytes are allogeneic to the graft recipient.