POLY(ETHYLENE GLOCOL) MODIFIED HUMAN GM-CSF WITH INCREASED BIOLOGICAL ACTIVITY

摘要

The present invention provides a full length, non-glycosylated human GM-CSF polypeptide having a single polyethylene glycol molecule covalently attached through an N-terminal amino acid. According to the present invention, the human GM-CSF polypeptide with the attached polyethylene glycol molecule has an unexpectedly increased ability to stimulate the replication of cells in culture compared to the non-modified GM-CSF molecule. This increased ability to stimulate cell division in culture is reflected in neutrophil-increasing activity in an animal model.