COMPOSITIONS AND METHODS EMPLOYING 5'-PHOSPHATE-DEPENDENT NUCLEIC ACID EXONUCLEASES

摘要

The present invention relates to compositions and methods employing 5'- phosphate-dependent nucleic acid exonucleases. In particular, the present invention provides kits and methods employing 5 '-phosphate-dependent nucleic acid exonucleases for selective enrichment, isolation and amplification of a particular set of desired nucleic acid molecules from samples that also contain undesired nucleic acid molecules for a variety of uses. In preferred embodiments, the desired nucleic acid molecules comprise prokaryotic and/or eukaryotic mRNA.