| 摘要 |
The present invention describes isolation of plasmid DNA or nucleic acid material from Escherichia coli (E. coli) or other species of microorganisms, or from other sources. The addition of indicator dyes to the alkaline lysis based purification buffers allows for increased ease of visual monitoring of the mixing and lysis steps of the procedure. The yield and quality of plasmid DNA is improved and the chance of error is reduced. The practice may be applied to other nucleic acid purification procedures.
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