GERMLINE AND SEQUENCE VARIANTS OF HUMANIZED ANTIBODIES AND METHODS OF MAKING AND USING THEM

摘要

The present disclosure provides methods of designing humanized monoclonal antibodies having minimal immunogenicity and retained antigen binding affinity, by using human germline sequences as templates for each of the variable light chain and variable heavy chain regions (CDRs and frameworks). The present disclosure also provides humanized COL-1 antibodies generated by the disclosed methods. In several embodiments, methods are disclosed for the use of a humanized COL-1 antibody in the detection or treatment of a tumor in a subject. Also disclosed is a kit including the humanized COL-1 antibodies described herein.