| 摘要 |
FIELD: biotechnology, peptides. ^ SUBSTANCE: invention relates to preparing conjugates of polypeptides with polyethylene glycol. Mutant peptide possessing the G-CSF activity is prepared by changes of above 15 amino acid residues in definite positions of amino acid sequence in G-CSF of wiled type with lysine residue mainly. Then the prepared mutant peptide G-CSF is conjugated with a polyethylene glycol (PEG) molecule. Invention provides increasing a half-life value and enhancing the biological activity of G-CSF in vivo as compared with the native non-conjugated G-CSF of wild type. Invention can be used in preparing conjugates of mutant form of G-CSF with PEG. ^ EFFECT: improved preparing method. ^ 15 cl, 3 dwg, 14 ex |
