Purification of alpha-1 proteinase inhibitor

摘要

A method for purifying human or other alpha-1 proteinase inhibitor (± 1 -PI) from a solution (which may be derived from the milk of a transgenic animal expressing the ± 1 -PI) which comprises contacting the solution with a cation exchange substrate under conditions sufficient to bind non-tg-± 1 -PI contaminants to the substrate while not substantially binding tg ± 1 -PI to the substrate. Using the preferred embodiment, the purified tg ± 1 -PI contains as little as 40 pg non-± 1 -PI-whey protein per mg total protein.