| 摘要 |
Human granulocyte-colony stimulating factor isoforms are provided to increase half life of the human granulocyte-colony stimulating factor in a living body without decrease of its activity. The human granulocyte-colony stimulating factor isoforms are provided, wherein at least one amino acid is added before threonine(Thr) of the N-terminal region or after proline(Pro) of the C-terminal region of human granulocyte-colony stimulating factor represented by the amino acid sequence of SEQ ID NO:1; and at least one amino acids added is cysteine and at least one cysteine is coupled with polyethylene glycol. A pharmaceutical composition comprises the human granulocyte-colony stimulating factor isoforms and pharmaceutically acceptable carriers. Genes encoding the human granulocyte-colony stimulating factor isoform proteins are provided.
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