Optimization of Process Variables in Oxygen Enriched Fermentors Through Process Controls

摘要

Methods and systems are provided for controlling the addition of oxygen in fermentations to achieve a desired oxygen consumption and substrate yield in fermentation cell cultures. In one aspect, the invention provides a method for regulating the addition of oxygen (O₂) to a fermentor during a fermentation process, comprising measuring real-time dissolved oxygen (DO) in a fermentation broth, measuring real-time O₂ concentration in the fermentor exhaust, and providing the real-time DO measurement and real-time O₂ measurement to an adaptive controller configured to regulate O₂ flow into the fermentor responsive to the real-time DO measurement and real-time O₂ measurement.