发明名称 |
Bacteria with reduced genome |
摘要 |
The present invention provides a bacterium having a genome that is genetically engineered to be at least 2 to about 20% smaller than the genome of its native parent strain. A bacterium with a smaller genome can produce a commercial product more efficiently. The present invention also provides methods for deleting genes and other DNA sequences from a bacterial genome. The methods provide precise deletions and seldom introduces mutations to the genomic DNA sequences around the deletion sites. Thus, the methods can be used to generate a series of deletions in a bacterium without increasing the possibility of undesired homologous recombination within the genome. In addition, some of the methods provided by the present invention can also be used for replacing a region of a bacterial genome with a desired DNA sequence.
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申请公布号 |
US2006270043(A1) |
申请公布日期 |
2006.11.30 |
申请号 |
US20040896739 |
申请日期 |
2004.07.22 |
申请人 |
BLATTNER FREDERICK R;POSFAI GYORGY;HERRING CHRISTOPHER D;PLUNKETT GUY III;GLASNER JEREMY D |
发明人 |
BLATTNER FREDERICK R.;POSFAI GYORGY;HERRING CHRISTOPHER D.;PLUNKETT GUY III;GLASNER JEREMY D. |
分类号 |
C12N15/74;C12N1/21 |
主分类号 |
C12N15/74 |
代理机构 |
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代理人 |
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地址 |
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