发明名称 |
METHOD FOR REFOLDING HUMAN ACTIVIN A |
摘要 |
A method for industrially producing human activin A which comprises refolding a modified human activin A produced by a microorganism into natural-form human activin A having a biological activity. This method comprises the steps of: (a) solubilizing the modified human activin A with a modifier and then protecting the thiol groups with glutathione and/or sodium sulfite; and (b) dialyzing the protected modified human activin A with a refolding buffer and then adding a thiol compound thereto to conduct a disulfide bond interchange reaction. |
申请公布号 |
EP0943690(B1) |
申请公布日期 |
2006.11.29 |
申请号 |
EP19960942573 |
申请日期 |
1996.12.19 |
申请人 |
AJINOMOTO CO., INC. |
发明人 |
EJIMA, DAISUKE;ONO, KUNIO;SASAKI, MICHIRO;ETO, YUZURU;TSUCHIYA, SHIGEKATSU |
分类号 |
C12P21/02;C07K14/575;C12P21/04 |
主分类号 |
C12P21/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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