发明名称 |
Universal RT-coupled PCR method for the specific amplification of mRNA |
摘要 |
The invention relates to a novel Universal RT-coupled PCR strategy for the specific detection and accurate quantitation of mRNA. Claimed and disclosed are novel Universal reverse transcription (RT) primers, a specific primer mix containing the Universal RT-primers, a transcript specific forward primer and a reverse PCR primer identical to a unique tag sequence, and methods and kits thereof for avoiding the amplification of genomic DNA and/or pseudogenes.
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申请公布号 |
US7141372(B2) |
申请公布日期 |
2006.11.28 |
申请号 |
US20030342684 |
申请日期 |
2003.01.15 |
申请人 |
HEALTH RESEARCH INCORPORATED |
发明人 |
SPIVACK SIMMON D.;HURTEAU GREGORY J. |
分类号 |
C12Q1/68;C07H21/02;C07H21/04;C12N |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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