METHOD FOR GENERATING HISTOGRAM

摘要

Disclosed is a method for detecting and measuring transient state fluorescence from a solution or a surface for immunoassays. The fluorescence method generates a histogram of the intensity as a function of time for a transient state fluorescence determination. This method comprises a step of determining a preliminary histogram of the shape of the histogram and multiplying it by the ratio of the total number of events divided by the number of events comprising the preliminary histogram. The method utilizes a relatively high powered and relatively high repetition rate light source with high speed detection electronics to increase the sensitivity and accuracy of the fluorescence analysis.