A NOBLE GEOBACILLUS THERMODENITRIFICANS CBG-AL STRAIN EXPRESSING THERMOSTABLE L-ARABINOSE ISOMERASE ACTIVITY, THE SAID ENZYME AND TAGATOSE-PRODUCTION METHOD

摘要

A Noble Geobacillus thermodenitrificans CBG-Al strain is provided to be able to stably produce D-tagatose at high temperature of 70 more than deg.C. And a method for producing tagatose is provided to be able to economically and significantly increase the tagatose production amount by using environment-friendly and relatively cheap substrate. The Geobacillus thermodenitrificans CBG-Al strain has more than 2.8 unit/mg of thermostable L-arabinose isomerase activity at a temperature of 70 deg.C and is deposited as a deposition number of KCTC 10399BP. The thermostable arabinose isomerase enzyme is produced by the Geobacillus thermodenitrificans CBG-Al strain and has a molecular weight of 56kDa, an isoelectric point of 5.3, an optimum enzymatic reaction pH of 8.0 and maximum thermostability at 60 deg.C. To produce tagatose from galactose, the Geobacillus thermodenitrificans CBG-Al strain is used.