| 摘要 |
The binding reaction between an affinity substance to be measured and a binding partner having the activity to bind to the affinity substance is measured using agglutination reaction. The binding partner is bound to carrier particles, and the carrier particles agglutinate through a binding reaction. According to the present invention, the agglutination reaction is enhanced by incubating the reaction solution before an electric field is applied. According to the present invention, the agglutination reaction is also enhanced by adjusting the temperature and/or viscosity of the reaction solution placed in an electric field. The present invention contributes to the improvement of measurement sensitivity. The present invention also comprises the step of diluting the carrier particle-containing reaction solution before counting the agglutinates. The binding between the affinity substance and the binding partner thereof is enhanced in the dilution step of the present invention. As a result, the disruption of agglutinates is prevented and the measurement sensitivity is increased. Furthermore, diluted carrier particles and agglutinates can be distinguished with high accuracy.
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