| 摘要 |
PROBLEM TO BE SOLVED: To provide a technique for purifying an RNA from a nucleic acid-containing sample by safe and simple operation. SOLUTION: As a result of intensive studies, it is found that a DNA is insolubilized and the RNA maintains a soluble state when an organic solvent is added to a mixture liquid of the sample containing the DNA and RNA with a chaotropic agent. A method for purifying the nucleic acid comprises mixing the nucleic acid-containing sample with the chaotropic agent and organic solvent, insolubilizing the DNA, separating the resultant insolubilized material from the mixture liquid and purifying the RNA from the residual liquid. The RNA can be bound to the solid phase containing silica by bringing the residual liquid into contact with the solid phase containing the silica even without adding a reagent, etc., to the residual liquid. Furthermore, the DNA can be purified from the insolubilized material. The high-purity RNA can be purified from the sample containing the DNA and RNA by the safe and simple operation. The RNA and DNA can simultaneously be purified from the single sample. COPYRIGHT: (C)2007,JPO&INPIT
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