发明名称 |
Peptide conjugates and fluorescence detection methods for intracellular caspase assay |
摘要 |
Polypeptides labelled with a donor and acceptor pair of dyes selected from a dibenzorhodamine dye and a diamino-benzophenoxazine dye are peptide conjugates which are useful for intracellular and bead-based assays with fluorescence detection. Peptide conjugates with a caspase-recognition site undergo cleavage into peptide fragments which may be detected, located, and quantitated by the changes in fluorescence. Intracellular cleavage of peptide conjugates is correlated with apoptosis.
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申请公布号 |
US2006252109(A1) |
申请公布日期 |
2006.11.09 |
申请号 |
US20050320162 |
申请日期 |
2005.12.27 |
申请人 |
APPLERA CORPORATION |
发明人 |
YAN XIONGWEI;MIRAGLIA SHERI;YUAN PAU M. |
分类号 |
C12Q1/37;C07K5/113;C07K7/06;C07K14/47 |
主分类号 |
C12Q1/37 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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