发明名称 |
METHOD OF PRODUCING HUMAN IGG ANTIBODIES WITH ENHANCED EFFECTOR FUNCTIONS |
摘要 |
A method for generating human IgG1 antibodies with enhanced Fc effector function is disclosed. In practicing the method, an IgG1 Fc look-through mutagenesis (LTM) coding library directed at four receptor-contact regions o f the Fc CH2 portion of in human IgG1 Fc is expressed in a system in which the mutated Fc fragments are displayed on the surfaces of the expression cells. The fragments are then screened for altered binding affinity to a selected F c receptor or other Fc-binding protein. The selected mutations may be used, in turn, to guide the selection of multiple substitutions in the construction o f a walk-through mutation (WTM) library, for generating additional Fc fragment mutations with desired binding properties. The antibodies so produced have a variety of therapeutic and diagnostic applications.
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申请公布号 |
CA2605697(A1) |
申请公布日期 |
2006.11.02 |
申请号 |
CA20062605697 |
申请日期 |
2006.04.26 |
申请人 |
BIOREN, INC. |
发明人 |
TAKEUCHI, TOSHI;RAJPAL, ARVIND;BHATT, RAMESH;SHEN, RANDY;CREA, ROBERTO;CAPPUCCILLI, GUIDO |
分类号 |
C07K16/00;C12N15/10 |
主分类号 |
C07K16/00 |
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