| 摘要 |
The proteins in a biological sample that is sought to be analyzed for its protein composition by a chromatographic procedure are coupled to a dye in a n unusually efficient manner by combining the sample with a dry powdered mixtu re containing the dye, a buffering agent, and in preferred embodiments, a denaturing agent as well. The dry powdered form of the mixture avoids deterioration or decomposition of the dye, and the combination of components in the mixture allows the dye to couple to the proteins in a relatively uniform and highly controllable manner when the powder mixture and sample ar e heated together and held at an elevated temperature until some or all of the powder dissolves. |
