| 摘要 |
PROBLEM TO BE SOLVED: To provide a shading correction method for a scanning fluorescence microscope equipped with functions of a confocal laser scanning microscope and a scanning cytometer for ensuring high-accuracy, high-performance measurement and image analysis. SOLUTION: A calibration sample 100 is prepared, has fluorescence beads 102 distributed and having the same diameter as a measured sample on a glass slide 101, and is two-dimensionally scanned by a laser light. An illuminance calibration coefficient map corresponding to a scan range is created from locations of the fluorescence beads in a scanned image acquired by scanning and their brightness data. COPYRIGHT: (C)2007,JPO&INPIT
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