发明名称 |
A METHOD FOR DETERMINATION OF DNA FRAGMENTATION IN ANIMAL SPERM CELL |
摘要 |
The present invention describes a method for the determination of DNA fragmentation in animal sperm. It particularly refers to a procedure to evaluate the integrity of the chromatin/DNA of the sperm by means of a treatment of the sample with a denaturing solution followed, optionally by a stain; a subsequent treatment with a lysis solution that does not contain a protein denaturing detergent, followed, optionally, by a stain; and an evaluation of the integrity of the chromatin/DNA. The present invention also refers to a Kit to evaluate the quality of the sperm of animals which includes a DNA denaturing solution and a lysis solution that does not contain a protein denaturing detergent.
|
申请公布号 |
EP1710320(A1) |
申请公布日期 |
2006.10.11 |
申请号 |
EP20050718163 |
申请日期 |
2005.01.26 |
申请人 |
UNIVERSIDAD AUTONOMA DE MADRID |
发明人 |
GOSALVEZ BERENGUER, JAIME;FERNANDEZ GARCIA, JOSE LUIS;GOYANES VILLAESCUSA, VICENTE |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|