RECOMBINANT PLASMID DNA pCL1 ENCODING POLYPEPTIDE WITH PROPERTY OF HUMAN LIGHT CHAIN ANTIBODY AGAINST EBOLA VIRUS, RECOMBINANT PLASMID DNA pCH1 ENCODING POLYPEPTIDE WITH PROPERTY OF INDICATED ANTIBODY HEAVY CHAIN AND THEIR USING

摘要

FIELD: biotechnology, genetic engineering, virology, medicine. ^ SUBSTANCE: invention reports about the construction of recombinant plasmid DNAs pCL1 and pCH1 in vitro comprising artificial genes encoding light and heavy chains of human full-scale antigen against Ebola virus prepared by genetic engineering methods and created on basis of variable fragments of recombinant antibody 4d1 light and heavy chains from phage library of human single-chain antibody, and human constant genes IgG1, cytomegalovirus promoter and polyadenylation BGH site. The combining use of plasmid DNA pCL1 and pCH1 provides the biosynthesis of human recombinant full-size antibodies of class IgG1 interacting with Ebola virus. Using recombinant full-size antibodies raised against Ebola virus can be used as a basis for the development of preparations used in diagnosis and treatment of dangerous diseases caused by this infectious agent. ^ EFFECT: valuable medicinal properties of plasmid. ^ 4 cl, 7 dwg, 6 ex