发明名称 |
VERFAHREN ZUR GEWINNUNG VON NUKLEINSÄUREN MIT HILFE CHAOTROPER-UND AMMONIUMREAGENTIEN |
摘要 |
A process for isolating nucleic acid from a nucleic acid-containing sample, which comprises: (a) providing a chaotrope; (b) providing a nucleic acid binding solid phase capable of binding nucleic acid in the presence of the chaotrope; (c) providing a source of NH4+ or NH3; (d) contacting the sample with the nucleic acid binding solid phase in the presence of a liquid phase comprising the chaotrope and the NH4+ or NH3; and (e) optionally separating the solid phase with the nucleic acid bound thereto from the liquid phase. |
申请公布号 |
DE60303020(T2) |
申请公布日期 |
2006.09.28 |
申请号 |
DE2003603020T |
申请日期 |
2003.04.02 |
申请人 |
QIAGEN AS |
发明人 |
REITAN, EVY;DEGGERDAL, ARNE;SKAGESTAD, VIDAR |
分类号 |
C07H21/00;C07H21/02;C01B21/068;C07H21/04;C12N1/08;C12N15/10;C12Q1/68;C12Q1/70;G01N33/553 |
主分类号 |
C07H21/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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